FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n02_Sample_178.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n02_Sample_178.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14075302
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA6408.458301E-1015.858072145
GTATTAG97700.014.1928461
GTATATA245050.013.953941
GTATAGG51300.013.8688151
TATACTG129550.013.4900365
GTCCTAT60550.012.7093221
CTATACT94500.012.6620534
GTATAGA91600.012.2847661
GTATTAT157500.012.2150711
GTCCTAC43400.012.2113531
GTATTAA141300.012.1769041
CCTACAC68500.012.175293
TCTATAC86300.011.8489633
GTATAAG88500.011.6486541
GTGCTAG76150.011.5357591
GTGTATA168250.011.4777621
TACACTG125150.011.4148155
TAATACT136200.011.3943254
CCTATAC55400.011.2579863
TATACAG123450.011.2195585
Produced by FastQC (version 0.11.5)