FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n02_Sample_168.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n02_Sample_168.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21363562
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC18450.022.397068145
GTATATA295250.014.1849051
TATACTG173200.013.3545635
GTATAAG136000.013.2358961
GTGTATA224750.013.1765291
GTATTAG150650.012.9605581
TATACAG181900.012.8354195
GTATAGG90600.012.8183561
GTATTAT206100.012.64321
GTATAGA137600.012.396241
GTATAAT199200.012.3888251
TATATAC300350.012.289323
CTGTCGC67900.011.7463989
GTGTAAG151300.011.6575661
ACTGATC176050.011.6552348
GGATCGT37000.011.5616798
GTATTAC104300.011.552191
CCTATAC81300.011.5063313
CTATACT133350.011.3649794
GTATATG206850.011.2639331
Produced by FastQC (version 0.11.5)