FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n02_Sample_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n02_Sample_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11880495
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG101500.016.2165785
GTATAGA76100.015.261511
GTATAGG49350.014.2674631
GTCTTAG72650.013.7881381
TATACGG11300.013.4753565
GTATATA188550.013.2432351
GTATAAG75650.013.2413531
TATACAG102300.013.0419235
TCTATAC72400.013.02127553
ATACTGT140550.012.7938166
GTGTAGG61500.012.6290551
TACACTG108750.012.6017815
GTATTAT125200.012.5810691
TAATACT116600.012.5001494
CCTATAC44150.012.4833643
GTGTATA128300.012.4468141
CTAATAC80650.012.3187023
GTCCTAA62050.012.2831491
TTATACT123800.012.2417454
TACACCG11901.8189894E-1212.1865965
Produced by FastQC (version 0.11.5)