FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n02_Sample_144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n02_Sample_144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15167599
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC14150.031.764841145
GCCGACC14500.019.998777145
TATACTG138300.014.0516435
GTATAGA98800.013.8866651
CCTATAC55800.013.77613
CTATACT99550.013.5491934
GTATTAA140600.013.3723441
GTCCTAG73000.013.325241
GTATATA259500.013.2317381
CTGCCGC43550.013.150745145
CCTACAC66800.013.1360123
GTATTAG99000.012.9053741
GACGACA19250.012.804412145
GTCCTAT68250.012.6571931
TACACTG135150.012.5549315
TATACAG131800.012.543945
GTACGGA20950.012.4741691
GTATAAG93200.012.4622711
GTGCTAG81300.012.41130351
GTGTATA176150.012.2395921
Produced by FastQC (version 0.11.5)