FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n02_Sample_141.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n02_Sample_141.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22722785
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC25450.020.224316145
GTGTATA248250.015.3231091
GTATAGG93700.014.8753251
TATACTG208300.014.5159255
GTATTAG164800.014.4925081
GTATATA370800.013.8611221
GTATAGA161250.013.4609681
GTATAAG150900.013.0372161
TATACAG203150.012.67095855
GTATTAT242950.012.6394541
TCTATAC150700.012.6077223
TACACTG204750.012.46575
GTCTTAG131300.012.4400641
GTATAAT229100.012.0727081
GTATTAA220200.012.0002111
CTATACT155200.011.9612564
CTAATAC166000.011.6641133
GTATACG25550.011.6492441
GTACTAG76800.011.62649251
ACTGATC167900.011.4869058
Produced by FastQC (version 0.11.5)