FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n02_Sample_135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n02_Sample_135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11757390
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA4103.8198777E-1122.985651145
GTATATA236000.014.7339651
GTATTAG86700.013.4804041
TATACTG126750.013.10106855
GTATTAT143950.012.6073691
CTAATAC89350.012.5807953
CCTATAC48650.012.5218193
GTATTAA125600.011.9640111
GTATAGA80950.011.8373151
GTGTATA161550.011.6832191
ACTGATC94100.011.6349378
TCTATAC82950.011.5406033
TATACAG114250.011.4879265
GTATAAG75850.011.4847561
TAATACG18100.011.2182834
CCTACAC51950.011.1679993
TCTAGAC80400.011.0948143
CTAGACT68800.011.0674554
GTCCTAG57900.011.0331681
ATTATAC127050.010.9596713
Produced by FastQC (version 0.11.5)