FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n02_Sample_116.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n02_Sample_116.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15081021
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA9300.019.487709145
GCCGACC14000.017.605753145
GTATATA207250.014.676651
GTATAGA89550.014.1866521
TATACTG117100.013.8708435
GTATAAG91100.013.8655891
GTATTAG94950.013.8385651
GTATTAT139000.013.4744911
TATACAG123900.013.10957055
GACTGCG14750.012.7809827
CCTATAC54600.012.618413
CTGCCGC47300.012.414453145
GTGTATA161250.012.3355431
GTATACG13650.012.2321451
GTACTAG48300.012.1743371
GTCCTAC44150.012.16771
ATAATAC141000.012.0871093
TCTATAC89700.012.0466823
CCGTATA15100.012.002363145
TGACGCG4907.249178E-411.8358145
Produced by FastQC (version 0.11.5)