FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84528870
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5740840.6791573103958446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC42100.014.739242145
TATACTG616100.014.5059765
GTATATA1142400.014.13071251
GTATTAG472550.013.2218281
GTATAGA455100.013.0392911
TATACAG619800.012.8584045
GTATAGG269700.012.8501061
CCTATAC273350.012.7321063
TCTATAC439100.012.6816533
GTGTATA764450.012.5334011
GTATTAT721050.012.456091
CTATACT442600.012.2978294
TACACTG617900.011.7489565
TATATAC1159800.011.5290513
CTAATAC487500.011.1084713
ACTGATC531300.010.899728
TAATACT668200.010.8436984
GATCGGA131350.010.837986140-144
GTCCTAG325600.010.8367081
GGATCGT123300.010.7256188
Produced by FastQC (version 0.11.5)