FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n01_Sample_81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n01_Sample_81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20501312
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG181250.012.9995335
CCTATAC81950.012.9159483
TATACAG185000.012.892785
GTCCTAG99400.012.8366231
GTATATA353050.012.7520481
GTATTAG135550.012.4618041
GTATAGA126400.012.2741441
GTCCTAT88600.012.19205951
GTGTATA251050.012.04204751
GTATTAT204050.011.7602331
GTATAGG79450.011.679941
CCGTACG4350.00401209711.6662474
GTCCTAC67100.011.5607441
CCTACAC99800.011.4049013
TAATACT191850.011.2987874
ATGCCGG28900.011.288907145
CTATACT134950.011.2278294
TACACTG194700.011.170635
GTATAAG129200.011.0542231
TCTATAC125900.010.9984143
Produced by FastQC (version 0.11.5)