FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n01_Sample_72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n01_Sample_72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22161887
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGGC44050.018.597908145
ATGCCGG46850.015.784184145
TATACTG231100.013.9598855
GTATTAG155700.013.6428061
GTATATA420450.013.3287631
CCTATAC87550.013.0834633
CTATACT159400.012.8712254
CGTCTTG44850.012.285213145
GTATTAT253450.012.2140671
GTCCTAG115700.012.1560661
TCTATAC152750.012.0077343
GTATTAA220250.011.9485561
GTATAGA148100.011.8463611
CCTATCG31950.011.799512145
TATACAG208550.011.64545255
CTAATAC150600.011.5533533
TATACCG16400.011.4934725
GTATAAG138250.011.3269591
GTATTAC115500.011.1728181
GTGTATA269400.011.0872621
Produced by FastQC (version 0.11.5)