FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n01_Sample_54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n01_Sample_54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8413447
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGC15850.016.46647145
GTATATA143500.014.7016391
GTATTAA76200.014.2712161
CTATACT54250.014.0316614
CTATACG7254.9149094E-913.9994044
TCTCCGC20550.013.758784145
GTGTATA96150.013.7229281
TCTATAC51800.013.7156333
GTATTAG59150.013.6048241
GTATAGA55200.013.3963281
GTATACG7609.5296855E-913.3548541
GTATAAG53000.013.1316731
GTCCTAT38150.013.112311
TATACTG78550.013.1057445
GTATTAT85400.012.9035851
GTATAAT77200.012.4899021
TCTCGTT42050.012.4134865145
TTATACT85750.012.3434994
GTCTAAG41000.012.2008451
GTACTAT43800.012.0829631
Produced by FastQC (version 0.11.5)