FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n01_Sample_44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n01_Sample_44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16342563
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGC32950.014.301733145
TATACTG149750.013.5555335
CTATACT111850.013.4171344
GTATAGG62250.013.3932271
GTATATA298150.013.3737951
CCTATAC65250.013.3329463
CCTACAC78050.013.189883
TACACCG11650.013.0682915
GTCCTAG79950.012.7857851
GTATTAG111950.012.7575971
GTATAGA111350.012.6961241
TCTATAC103400.012.3400683
CTAATAC117350.012.1705043
GTCCTAC56200.011.997021
TATACAG146400.011.8849025
GTATTAA160150.011.8604491
TACACTG158850.011.5923625
ACTGATC139700.011.5726078
GTGTATA192600.011.5560351
GTCTACG12607.2759576E-1211.5076381
Produced by FastQC (version 0.11.5)