FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n01_Sample_35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n01_Sample_35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19742626
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC77350.015.9336463
GTCCTAG101650.014.9063011
CCTACAC98650.014.4775723
CGTACGG8802.7284841E-1114.0053145
TATACTG206050.013.897965
TCTATAC134200.013.3435383
TCGTACG8201.9408617E-913.2618464
ATGCCGG66400.013.10233145
GTCCTAC62700.012.9503491
CGTATAC19050.012.9392933
CTATACT137350.012.6152494
GTATATA372350.012.5585451
TATACAG182200.012.4941835
TACACTG189300.012.4851455
GTATTAG136700.012.2510811
GTATAGG73900.012.1648921
CCTAGAC113950.012.151943
TATACCG15600.012.08301455
GTATAGA131800.011.8814461
GTGTATA243300.011.6808621
Produced by FastQC (version 0.11.5)