FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n01_Sample_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n01_Sample_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26870761
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG218600.015.3220115
GTATATA356950.014.6437881
GTATAGG130600.014.5439941
GTATAGA191650.014.0342971
GTATTAG194300.013.9921361
GTATAAG192800.013.875381
TATACAG244150.012.97623355
GTATTAT298350.012.7816011
TTATACT270800.012.1543344
TATATAC368250.012.0681773
TACACTG218400.011.9169685
GTGTAGG147300.011.9107251
TAGACTG169450.011.8084115
GTATAAT260600.011.7676981
TAATACT264150.011.7467324
CTATACT178600.011.7311444
GTATATG267550.011.6516931
GTGTATA257350.011.6064281
TATACCG55650.011.5944135
CCTACAC144900.011.5575963
Produced by FastQC (version 0.11.5)