FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n01_Sample_223.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n01_Sample_223.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19070880
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG187700.015.179235
CACGAGC40900.014.712731145
GTATTAG129150.014.3143171
GTATATA334300.014.1612511
GTATAGA123050.013.4331561
TAGATCG16400.013.262211145
GTATAGG75800.012.9118781
GTATTAA173050.012.7777111
TCTATAC127650.012.6650083
GTCCTAT83400.012.6045341
GTGTATA218300.012.3873941
TATACAG167400.012.2127955
TATACCG12550.012.1310115
GTATAAG111600.012.0829671
TACACCG14500.011.9995425
TAATACT175100.011.9655634
GTATAAT189800.011.9174461
GTATTAT201700.011.897261
CTATACG16500.011.8631834
CTATACT131200.011.82500654
Produced by FastQC (version 0.11.5)