FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n01_Sample_221.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n01_Sample_221.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36659237
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG340750.015.3610495
GTATATA659000.015.0495591
GTATAGG153600.014.7260571
GTATTAG262000.014.5271731
GTATAGA245800.014.0983761
GTGTATA431700.013.6363171
GTATAAG233200.013.5855111
TATACAG342050.013.2255755
ATAATAC396650.013.0134383
GTATTAT399000.012.9732391
TCTATAC233950.012.643193
TATATAC647500.012.6183823
ACTGATC327100.012.5888878
TAATACT358200.012.4673654
CCTATAC146100.012.3557383
ATACTGT454750.012.1638586
CTATACT248050.012.0414154
TACACTG345150.011.9725465
GTATTAA352250.011.9371351
CTAATAC256200.011.9130383
Produced by FastQC (version 0.11.5)