FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n01_Sample_205.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n01_Sample_205.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16592409
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG114700.014.7904711
CCTATAC63950.014.1707813
TATACTG156500.013.943655
CACGAGC31350.013.875287145
GTATAGA104300.013.4848781
GTCCTAG80850.013.3609041
GTATAGG62950.012.553371
GTATATA306750.012.40807151
CTATACT110800.012.36648754
TATACAG147450.012.1935825
GTCCTAT76350.012.05936051
TCTATAC104050.011.9842453
GCGCTAG9159.91713E-911.88501551
GTGTATA199950.011.7477091
TACACTG155900.011.5791735
GTCCTAA89050.011.5606971
GTATAAG106050.011.4849251
ATAATAC184800.011.3375763
CGTCTTG68700.011.186067145
TAATACT163000.011.1637584
Produced by FastQC (version 0.11.5)