FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n01_Sample_184.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n01_Sample_184.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14198252
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG122650.014.7181865
GTATAGG56600.014.2179411
GTATATA222450.014.0467231
GTATTAG99950.013.8541771
GTGTATA142500.013.4822131
GTATAGA97900.013.3296881
GTATAAG90450.013.0649941
CCGATCG5107.079852E-512.7938879
TACACTG125050.012.5225455
TATACAG131500.012.4596335
GTATAGC50800.012.2734011
GTATTAT150150.012.0710451
GTACTAG47600.012.0323461
TATATAC226000.012.0294393
GTATATG135100.011.96684551
GTATAAT140250.011.8375761
GGATCGT27800.011.7351118
ACTGATC112300.011.684728
GTCCTAT63400.011.5494761
ATAATAC151000.011.5227693
Produced by FastQC (version 0.11.5)