FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n01_Sample_178.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n01_Sample_178.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14075302
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA245850.015.4520271
TATACCG8601.8189894E-1114.3308455
TAATACT134850.013.5478664
TATACTG129750.013.521645
CCTATAC52600.013.2314313
GTATTAG93000.012.8625011
GTATAAG86500.012.7394861
GTCCTAG72250.012.6432021
ATGCCGG21350.012.564187145
CTCCGAC15800.012.3890295145
TCCGTAC17000.012.3671723
TATACAG121700.012.33107955
GTCCTAC45700.012.2151541
GTGTATA167500.012.2055881
GTATAGG52400.012.1751921
TGCGTTC44900.012.110037145
ACTCGCG12103.6379788E-1211.9829258
ATAATAC154700.011.8095013
GTATTAT152950.011.8025071
CTATACT95200.011.7274914
Produced by FastQC (version 0.11.5)