FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n01_Sample_168.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n01_Sample_168.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21363562
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA135450.015.2008061
GTATAGG85050.014.9173271
GTATTAG150650.014.822071
GTATATA291600.014.3703581
GTATAAG134100.013.51571
TATACTG163300.013.4962365
TATACAG181150.013.0468165
TACACCG21750.012.9996185
GTGTATA225200.012.9737031
GTATACG21350.012.9036631
GTATTAT196950.012.66281
GTATTAA188800.012.6334281
CTAATAC146750.012.251773
GGATCGT40950.012.2156448
TCTATAC129500.012.2042753
TATATAC295900.011.9808923
ACTGATC177750.011.746388
TATACTA150800.011.49004655
TACACTG193150.011.4855555
GTATAAT193450.011.3553671
Produced by FastQC (version 0.11.5)