FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n01_Sample_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n01_Sample_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11880495
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT119850.014.3968341
TATACTG103900.014.3739495
GTATAGG46000.014.3420641
TATACAG103350.014.3101465
GTATATA187400.014.1592261
GTATTAG80850.013.9885481
GTCCTAG58000.013.8746821
CTATACT77450.013.5728484
CTAATAC77350.013.4966693
CACGAGC28650.013.411956145
GTGTATA127900.013.2639661
GTATAGA75750.013.2076191
GTATAAG78700.013.0810221
TACACTG111200.012.9739655
ATAGGAC52300.012.7529473
GTGTAGG58100.012.7277631
GTCTAAG63600.012.6530121
GTGTTAG95450.012.5324541
GTATACG13900.012.5176991
GTCTTAG69550.012.50871