FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n01_Sample_144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n01_Sample_144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15167599
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC18000.021.347347145
CACGAGC28100.016.770563145
CTGATCG19050.015.603767145
GTATTAG99250.014.6089831
TATACTG131700.014.2021165
CCTATAC54400.013.9929573
CCTACAC71400.013.9104583
TCTATAC91350.013.5708393
GTCCTAT65600.013.3721761
ATGCCGG23300.013.068747145
GTATATA258600.013.0360191
GTCCTAC45900.012.7936031
CTATACT94500.012.5047464
TATACAG134550.012.5003845
CTATACA111850.012.4446974
TACACTG135800.012.3319385
GTGTATA174050.012.2043341
TAGACTG108250.012.1888465
GTATTAT158600.012.113321
CTACACT99600.012.0828084
Produced by FastQC (version 0.11.5)