FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n01_Sample_141.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n01_Sample_141.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22722785
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG201400.015.4427625
GTATAGG92700.014.8594261
GTATATA379350.014.5436271
GTGTATA252650.014.4623671
GTATAGA160200.014.30054951
GTATTAT242850.014.2996551
CTATACT153050.013.6422614
GTATAAG141600.013.5166421
TATACAG208150.013.5139565
GTATTAG155400.013.4359871
TCTATAC149500.013.2872933
GTATTAA210550.013.0500491
CCTATAC94150.012.3204573
CACGAGC53250.012.253298145
GTATTAC123850.011.7659841
GTCCTAG104150.011.6944061
GTGTAGG110700.011.6573681
CTAATAC160550.011.6502873
ATACTGT269700.011.6394966
TATATAC380050.011.5218823
Produced by FastQC (version 0.11.5)