FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n01_Sample_135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n01_Sample_135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11757390
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACG5150.022.523655145
AGACGGC27250.020.21963145
ATGCCGG27500.017.663153145
GTATATA238250.014.6364731
TATACTG122000.014.4399945
GTATTAG85800.014.279851
GTGTATA150050.012.7070281
CCTATCG21750.012.66632145
TATACAG113200.012.6165445
TAGTCCG6504.041827E-612.2687385
TCTATAC83900.012.183663
CCTATAC46750.012.0957723
CACGAGC17650.011.911856145
CTAATAC85800.011.8293623
CTATACT89800.011.7868324
GTATAGA84900.011.6986881
ATAATAC138300.011.6896973
GTATTAA117500.011.6613331
GTCTATA72550.011.3917831
TATACGG10204.0563464E-911.3720925
Produced by FastQC (version 0.11.5)