FastQCFastQC Report
Thu 7 Feb 2019
HLYKLBGX9_n01_Sample_116.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYKLBGX9_n01_Sample_116.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15081021
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGC41000.018.92033145
CGAGACG8300.016.596035145
TATACCG9800.016.2750035
CCTATAC52750.015.5303223
GTATTAG97700.014.8409871
GTATATA209550.014.2194081
GTATAAT135550.013.8524821
CTCCGAC15250.013.7865925145
GTGTATA155250.013.5890391
TCTCCGC34350.013.50772145
GTATAGG53850.013.4629951
ATAATAC137450.013.3444393
GTATTAT134600.012.8730131
GTATAGA88450.012.8685371
TATACTG114400.012.4843145
GTATAAG87300.012.3736941
TATACAG112850.012.2703315
GTATTAC70400.012.15169051
GGATCGT25800.012.0828778
TAATACT129350.011.9381684
Produced by FastQC (version 0.11.5)