FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25120333
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25106469.99447738212706No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG915100.3642865721565076No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC522800.20811826021573837No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA351010.13973142792334797No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA299280.11913854804392919No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA292080.11627234400117228No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA280990.11185759360753697No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA836850.069.4409261
AGTAGGT612950.069.433647
TAGTAGG613150.069.344466
TAGGTTG613550.069.231159
GGTAGTA842350.069.223544
TATAGTT565500.069.125216
GTTGTAT473950.069.08131412
AGGTAGT842100.069.0706253
TGTATAG614350.069.0537614
GTAGTAG837250.069.015045
GAGGTAG880700.068.952872
CGGAGTC401450.068.8214714
AAGATGC185950.068.7553255
TTGTATA617200.068.749113
TAGTTAA560550.068.5647718
GTATAGT617600.068.52888515
GCATAGC186100.068.2280414
GGCATAG187600.068.1698713
TCCACCG406400.068.162739
ATAGTTA566400.068.1085617
Produced by FastQC (version 0.11.5)