FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7567803
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG914401.2082766953632382No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC491200.6490655213937255No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA205400.27141298471960756No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC188430.24898903948741796No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT176210.23284168470030206No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG149310.19729636196925315No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT147440.19482536741508732No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA147360.19471965641811767No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC145510.1922750896131942No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG126050.1665608896003239No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA113650.15017568507002627No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT109560.14477121034995227No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT585800.069.5489048
CCTTCAT420350.069.546982
AGATGCT193050.069.534586
AGTAGGT586600.069.5256357
AGGTTGT612300.069.4658310
AAGATGC193350.069.4628755
GTTGTAT445400.069.4525712
TGAGGTA808200.069.452541
TAGGTTG586600.069.412769
TAGTAGG588300.069.410756
TATAGTT519350.069.3404316
AGGTAGT800100.069.336063
TGCTGGC194650.069.284329
CGGAGTC421900.069.2822714
GGTAGTA802700.069.2684254
TGTATAG577250.069.2598314
GCTGGCA194900.069.1779510
GAGGTAG838750.069.166292
GTAGTAG797850.069.158795
ACAGTGG171650.069.1518864
Produced by FastQC (version 0.11.5)