FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7625716
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG1019611.337067889756188No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA394950.5179185797110724No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC346480.4543573351013859No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA235300.3085611895328911No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA185880.243754160265082No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC179870.23587293311211696No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT164510.21573056221868217No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160340.2102622232456598No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG139470.18289430133511397No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT119800.15710000215061773No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA116680.15300858306288878No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA106240.13931806534625732No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT103350.13552825728102122No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC100840.13223676307903415No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC84910.1113469213907258No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA81310.10662605321257702No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT611750.069.6143657
GTAGGTT611550.069.5970848
TAGGTTG611450.069.5684059
AGGTTGT639250.069.5044610
TGAGGTA834350.069.498751
GGTAGTA825800.069.4605944
TAGTAGG613300.069.4605566
GTTGTAT460450.069.431412
GTTGTGT183850.069.4151812
AAGATGC199800.069.394795
AGATGCT199850.069.378346
CGGAGTC438800.069.36323514
CCTTCAT440550.069.316022
TATAGTT532850.069.3097616
AGGTAGT826200.069.29993
GTAGTAG823350.069.259255
TGTATAG592700.069.2413714
CTTCATT440750.069.204263
CAAGATG200400.069.15214
GGAGTCT438850.069.14802615
Produced by FastQC (version 0.11.5)