FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6974424
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG810491.1620887975838579No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC379370.5439445608698296No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA331090.4747202062851355No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA203630.29196676313341435No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC195350.2800948149983425No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT148650.21313588046840856No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT147060.2108561223120361No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140780.20185179449944537No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG129330.18543466815324103No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA113950.1633826678733613No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT113600.1628808343169271No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC107780.15453605917850707No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC81110.11629634217822146No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA75340.10802325754786346No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA72210.1035354317431805No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT535850.069.632338
CCTTCAT421300.069.5351262
TAGGTTG536300.069.514189
AGTAGGT537050.069.50437
AGATGCT186950.069.485576
TAGTAGG537000.069.4696356
CGGAGTC420200.069.417214
GGTAGTA739050.069.403334
TGAGGTA747800.069.337191
AGGTTGT563100.069.33147410
GTTGTAT407350.069.32252512
AGGTAGT738500.069.299633
AGCTTAT452300.069.295632
TATAGTT479250.069.2898416
GGAGTCT420150.069.28387515
CTTCATT422800.069.271383
GTTGTGT160500.069.26449612
AAGATGC187500.069.243935
GCTGGCA187300.069.2179910
GTTGACA281900.069.20244618
Produced by FastQC (version 0.11.5)