FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7076202
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG947591.3391223144845215No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA386550.5462676164417013No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC333070.47069035055811015No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA228280.3226024356003404No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT185790.2625561000095814No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC163330.23081590943842475No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG158160.22350973021968568No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT153660.2171503866056961No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA127880.18071841363488492No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG127560.1802661936445568No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT111600.15771172162694055No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC103890.1468160462349718No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA103850.1467595187361808No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA102780.14524740814352105No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC72410.10232890468644056No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT582950.069.593858
AGGTTGT610000.069.5131410
AGTAGGT584250.069.504397
TAGGTTG583850.069.444629
TGAGGTA799500.069.443871
GTTGTAT437300.069.43841612
CCTTCAT363050.069.427932
TAGTAGG585450.069.4211046
AGATGCT176000.069.4123466
GGTAGTA792100.069.387144
AGGTAGT791350.069.3772353
GTTGTGT178550.069.2966212
CGGAGTC363900.069.2623414
TATAGTT511450.069.2504916
GTAGTAG789450.069.2442555
CTTCATT364000.069.218383
TGTATAG565750.069.1980214
AAGATGC176650.069.1965565
GCTGGCA176400.069.1702310
CAAGATG176550.069.1554954
Produced by FastQC (version 0.11.5)