FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4594579
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG527471.1480268377146199No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA443910.9661603380853827No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA216210.4705763030736875No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC193100.4202778970608624No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA135730.2954133556088599No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT131260.28568449905856447No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA127360.2771962349542798No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC100840.2194760390451443No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT92980.20236892215804758No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG87290.1899847624776938No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG81970.1784058996482594No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC68830.14980697905074652No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA60230.13108926846181118No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA56380.12270982825629943No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC53480.11639804212747239No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT50600.1101297855581545No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA47780.10399211766736409No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG47540.10346976295325426No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT46410.10101034284098716No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTTG344450.069.465439
GTAGGTT344850.069.4464958
AGGTTGT362150.069.4416810
CCTTCAT244450.069.398142
GTTGTGT104100.069.3769712
AGTAGGT345300.069.366137
GGTAGTA472250.069.3255844
TAGTAGG346050.069.288076
TGAGGTA477350.069.2841
CTTCATT245050.069.257543
GTTGTAT261200.069.2318112
TATAGTT308400.069.1877816
GGATAGG49300.069.13029514
AGATGCT112150.069.112166
TGCTGGC112050.069.1091169
GTAGTAG471300.069.073315
CGGAGTC245650.069.07036614
GCTTATC236550.069.068643
GGTTGTG104750.069.0481811
CCACCGG246250.069.016510
Produced by FastQC (version 0.11.5)