FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5738142
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG790031.3768045475347248No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA414410.7222024132550223No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC281810.4911171595265506No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA193730.33761799551143906No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT165410.2882640408689781No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT133570.23277569638395146No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA126190.21991439040720848No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC122860.2141111181981903No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG117620.20497924240982535No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG113990.19865315288467938No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT88980.1550676159634948No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC82760.14422787027577916No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA81030.14121295708610906No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA76280.13293501624741946No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC74110.12915330432742864No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA61820.10773522161006124No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGATGC147150.069.709275
GTAGGTT430550.069.5872658
AGTAGGT431850.069.524867
AGATGCT147800.069.5204856
CCTTCAT295300.069.4756242
AGGTTGT448450.069.4471110
TGAGGTA599250.069.441481
TAGGTTG431400.069.441459
GTTGTAT329050.069.42309612
TAGTAGG432000.069.410776
TATAGTT387950.069.3739816
GGTAGTA595150.069.3066564
AGCTTAT346200.069.289452
CAAGATG148050.069.28554
TGCTGGC148700.069.278949
GCTTATC346050.069.226043
TGTATAG427650.069.19301614
CTTCATT296050.069.178993
AGGTAGT595800.069.119443
GCTGGCA149150.069.1174510
Produced by FastQC (version 0.11.5)