FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6635655
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG780941.1768845728115762No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC466570.7031257652786349No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA349630.5268959884141053No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA192390.28993369908471733No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA186450.28098205828964884No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC183430.27643088738037164No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT158390.23869535109947698No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG146280.22044545715532224No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT132350.1994528045837223No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC130650.19689088718446032No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT129000.1944043202969413No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA116860.17610921604574076No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA113660.17128678329418875No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG109850.1655450742993721No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC71970.10845952660287492No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT481000.069.507278
AGTAGGT481400.069.47867
TAGGTTG481600.069.398879
TGAGGTA676200.069.3511051
AGGTTGT504050.069.3294710
TAGTAGG482850.069.3181466
CCTTCAT340200.069.2932052
TATAGTT442350.069.25329616
CGGAGTC338850.069.22426614
AAGATGC147950.069.211615
ACAGTGG137850.069.2046664
AGCTTAT387600.069.198162
GTTGTAT370000.069.1945512
GGTAGTA672600.069.185184
GGAGTCT338400.069.18188515
AGGTAGT671800.069.147763
TGTATAG487750.069.1418914
AGATGCT147850.069.140076
TGCTGGC148000.069.1125959
GTTGACA243950.069.08372518
Produced by FastQC (version 0.11.5)