FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7012238
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG928241.3237428621219076No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC458280.6535431341605918No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA375850.5359915051371617No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA224860.3206679522286608No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC203130.2896792721524854No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG169560.24180582575776807No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT154950.22097082272449967No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG140280.20005025499704945No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA134100.191237091496324No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT127730.1821529731306895No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT126460.18034185377050807No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC123970.17679091896196336No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA121980.1739530232716003No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC99180.14143843948251614No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA84800.1209314344436113No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA78200.11151931808361325No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG71870.1024922428474333No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT71700.10224980954725152No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT537600.069.458468
TGAGGTA746400.069.447111
CCTTCAT410100.069.4104162
AGTAGGT538450.069.40087
TAGGTTG538200.069.335519
TATAGTT485050.069.32445516
AGGTTGT564600.069.32252510
TAGTAGG538600.069.308466
AAGATGC180350.069.292635
GGTAGTA739700.069.281534
AGATGCT180350.069.2727366
GTTGTAT413300.069.2590412
CTTCATT411150.069.2421653
AGGTAGT739800.069.182283
GTTGTGT156400.069.1817212
TGCTGGC180800.069.1728069
GGAGTCT410450.069.160715
GTAGTAG736300.069.145175
AGCTTAT420000.069.14082
ACAGTGG156600.069.0973054
Produced by FastQC (version 0.11.5)