FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7353000
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG814271.1073983408132735No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA382310.5199374405004761No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC248660.3381748946008432No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA194670.2647490820073439No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT153590.20888072895416837No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG143800.19556643546851626No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA142000.19311845505235958No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT133950.18217054263565893No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG113510.15437236502107982No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC105030.14283965728274173No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA91950.12505099959200325No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT77800.10580715354277165No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT549300.069.633788
AGTAGGT550050.069.564287
TAGTAGG550700.069.524086
TAGGTTG550300.069.488179
AGGTTGT575850.069.46763610
GGTTGTG155500.069.44549611
TGAGGTA771850.069.420561
CCTTCAT372450.069.41532
GGTAGTA763650.069.414674
GTTGTAT424850.069.3998412
CTTCATT371750.069.375623
AGATGCT171150.069.354126
TGCTGGC171650.069.352199
TATAGTT508050.069.3376616
GTTGACA281100.069.3253318
GGAGTCT371250.069.2965615
GTTGTGT155550.069.28725412
GCTGGCA171700.069.2708610
TGTATAG560300.069.2601814
AGCTTAT447850.069.230492
Produced by FastQC (version 0.11.5)