FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7163143
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG860711.201581484552242No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC269660.3764548606666096No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA225790.3152107950378765No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA222380.3104503148966871No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT166420.2323281833128279No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT160950.22469187059367654No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG150060.20948904691697484No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG142560.19901878267682216No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC138280.19304375188377504No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA106360.14848230727768524No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC88750.12389812684180673No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT82570.11527062910792092No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC78890.110133219454086No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA76480.10676877454491696No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT579350.069.565217
GTAGGTT578800.069.558778
AGGTTGT602750.069.5003510
TAGGTTG579250.069.4503569
TAGTAGG580500.069.437316
CCTTCAT406750.069.4331742
TATAGTT517100.069.3982916
TGAGGTA802850.069.382651
AGATGCT183200.069.380656
GTTGTAT440550.069.3605112
GGTAGTA795300.069.348524
AGGTAGT795150.069.291183
TGCTGGC183150.069.242869
CGGAGTC407850.069.2315314
AGCTTAT464450.069.2313842
AAGATGC183850.069.211015
TGTATAG572850.069.19244414
GAGGTAG828450.069.164462
CTTCATT407650.069.158243
GTTGACA289000.069.15156618
Produced by FastQC (version 0.11.5)