FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5104416
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG572781.1221264097597061No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA500330.98019048604189No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA393630.7711557992138571No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA265220.5195893124698301No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG246040.48201400512810866No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC229460.44953232651884173No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA226790.44430156162820583No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT218380.4278256317666898No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA124510.24392604364534554No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC115460.22619629748045614No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC114500.22431557302539604No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG108340.21224759110542715No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA92850.18190131838784301No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA75850.14859682282948725No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA69220.1356080695617285No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC58670.11493969143580773No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG53610.10502670628726185No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT420950.069.5717248
AGGTTGT447700.069.5418410
AGTAGGT421700.069.522677
CCTTCAT236100.069.5043952
TAGGTTG422000.069.423499
TGAGGTA615800.069.38361
GGTTGTG125800.069.3760811
AGATGCT111350.069.3635946
TAGTAGG422450.069.362596
GTTGTAT326950.069.35589612
GGTAGTA605150.069.3380054
TATAGTT404150.069.31998416
TGTATAG446800.069.2426514
AGGTAGT605050.069.239573
GTTGTGT125450.069.2342212
GTAGTAG602900.069.2143555
CTTCATT236600.069.207563
GAGGTAG637250.069.158792
CGGAGTC237200.069.13103514
TTGTATA447350.069.0956213
Produced by FastQC (version 0.11.5)