FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7324062
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG843121.151164476761666No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA543750.7424158888878876No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC505730.6905048045742922No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA458640.6262098818934083No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA296650.4050348017261459No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA228140.3114938131326578No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC192690.26309171058355324No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG177620.24251569689060526No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT176490.24097283720427273No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA161000.21982337123852858No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG155420.21220464818566528No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT153390.20943296220048385No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC153270.20926911869397066No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA123830.16907284509606826No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA82740.1129700977408438No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC79070.10795921716664877No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT555300.069.5507668
CCTTCAT365700.069.5051652
TAGGTTG555800.069.475619
AGTAGGT556700.069.4457
TGAGGTA773350.069.435841
GTTGTAT431800.069.4326212
AGGTTGT583750.069.4091710
AAGATGC169900.069.393985
TAGTAGG557250.069.382156
GGTAGTA763200.069.370394
CTTCATT366650.069.303633
TATAGTT509450.069.2546616
CGGAGTC366750.069.22945414
AGGTAGT763750.069.166073
TGCTGGC170550.069.164889
TGTATAG560550.069.14046514
GTTGTGT158100.069.0983612
GTAGTAG760350.069.089215
AGATGCT170650.069.068496
GGTTGTG158500.069.0583311
Produced by FastQC (version 0.11.5)