FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6671791
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG905871.357761356733147No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC633170.9490255315251932No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA346050.518676319447057No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA312130.4678353983210805No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA299610.44906982248095006No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA263040.3942569543920066No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC248690.3727484868755631No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT201700.3023176235586516No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT199400.2988702733643785No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG197180.2955428310029496No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC190410.28539563064850204No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA150610.22574148380847064No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG135900.20369343104422785No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC105600.15827833935445518No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA104930.15727411125438434No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA85070.12750699175079075No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG79280.11882866234868568No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA78700.11795933056056462No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT73180.10968569009430901No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC72340.10842665784944402No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG67760.10156193441910874No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT484500.069.589038
TAGGTTG484600.069.537529
AGTAGGT485600.069.517877
AGGTTGT507600.069.4830210
TAGTAGG486550.069.458216
AGATGCT160450.069.44526
TGAGGTA677350.069.41451
GTTGTAT367950.069.3686212
AGGTAGT669200.069.362943
GGTAGTA670450.069.358894
CCTTCAT329050.069.342762
TATAGTT440100.069.2860516
GTTGTGT145350.069.26453412
AGCTTAT383500.069.142852
GTAGTAG668000.069.1417855
TGCTGGC161550.069.140469
TGTATAG483300.069.13914514
GAGGTAG703000.069.125332
GGATAGG80550.069.1174214
CGGAGTC329900.069.1164814
Produced by FastQC (version 0.11.5)