FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7430525
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA974151.3110109985499006No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG909161.2235474613166633No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA706390.9506596101890512No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA597830.8045595701515035No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC426260.5736606767354931No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA258370.34771432704956917No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA217320.2924692400604264No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT195440.263023137665239No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG184470.24825971246984568No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC174170.23439797322531045No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA151520.20391560488659954No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG143390.1929742514829033No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC129970.1749136164672079No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT117160.1576739194067714No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTAACTGTAGGCACCATCA107140.14418900414169927No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA105990.14264133422604727No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC90810.12221209133944101No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA75140.10112340648877434No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT468150.069.599488
TAGGTTG468550.069.4943169
GTTGTAT359100.069.47169512
AGGTTGT488050.069.4642910
AGTAGGT469750.069.436917
TGAGGTA647100.069.410871
AGATGCT155250.069.383786
TAGTAGG470100.069.374536
CCTTCAT339600.069.3328862
GGTAGTA641100.069.31464
TATAGTT424200.069.2869716
TGTATAG465150.069.1977814
GTTGTGT134650.069.05257412
CTTCATT340800.069.0262153
TGCTGGC156150.069.023599
CGGAGTC340750.069.01208514
GGATAGG74900.069.0065714
GTAGTAG639300.069.0015955
GGAGTCT340100.068.9690615
AAGATGC156200.068.96185
Produced by FastQC (version 0.11.5)