FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7447526
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG891061.1964510093687488No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA744210.9992714359103949No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA635360.8531155178243084No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA491400.6598164276297928No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC397010.5330763531406268No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA247360.3321371419180007No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA209840.28175799587675154No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT186140.24993534765773226No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG179680.24126132624444682No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG159290.21388310695390658No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC135390.1817919131802964No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA117250.15743483137890354No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT116820.15685745843653315No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC116230.15606524905049007No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA89150.11970418095888487No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTAACTGTAGGCACCATCA87510.11750210741123966No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC78810.10582037578653636No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT531700.069.616537
GTAGGTT531000.069.602878
AGGTTGT559050.069.4771410
TGAGGTA741650.069.448851
TAGGTTG532250.069.418759
TAGTAGG532950.069.4058466
CCTTCAT351400.069.395062
GTTGTAT413200.069.37533612
GGTAGTA733450.069.3413544
AGATGCT169550.069.288496
TATAGTT493450.069.22629516
AGGTAGT734100.069.194623
GTTGTGT152150.069.1801312
TGTATAG537950.069.1719814
AGCTTAT368750.069.1099852
GTAGTAG731000.069.0998155
GCTTATC369100.069.0900043
CTTCATT352650.069.087683
TGCTGGC169800.069.077399
GGAGTCT352600.069.05221615
Produced by FastQC (version 0.11.5)