FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7932891
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG1033221.3024507710997164No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA537630.6777226612593064No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA522590.6587636209800437No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC449860.5670820385657637No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA369740.4660848106951173No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA288140.3632219325842243No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG232280.29280624175978215No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT220940.278511327081136No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG215690.27189331102620723No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA166170.20946966244714568No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC161020.2029777038408822No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC142960.18021172861192725No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT141250.17805614623975044No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA105190.1325998302510396No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC104190.13133925576438654No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA85960.1083589828727005No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT568700.069.511538
AGTAGGT569850.069.4633567
AGGTTGT593800.069.4355210
TGAGGTA791650.069.410941
AAGATGC182600.069.395195
GTTGTAT434000.069.3707912
TAGGTTG569900.069.351139
CCTTCAT366850.069.331952
GGTAGTA784050.069.324254
TAGTAGG570150.069.289146
TATAGTT517050.069.2814716
AGATGCT183200.069.224786
CGGAGTC366750.069.1825414
AGGTAGT784550.069.164093
CAAGATG183300.069.1301654
GTAGTAG780650.069.128595
GCTGGCA183550.069.1256710
TGTATAG568350.069.1158814
GGATAGG95150.069.1013814
GAGGTAG820600.069.069022
Produced by FastQC (version 0.11.5)