FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6626426
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG787311.1881367120073476No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC389350.5875716411833468No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA363210.548123528429956No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA317870.4797005203106471No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA312540.4716569686283375No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA211600.31932749267855703No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT158430.23908816004283456No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG153540.23170861637932727No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG133730.20181316444188765No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC128630.19411670786031565No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC117120.17674686173210113No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT115330.17404555638288272No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA104460.15764154010019882No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA88770.13396361779336252No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC68310.10308724491905591No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT464950.069.511818
AGTAGGT465850.069.467667
TAGGTTG464700.069.450789
TGAGGTA645050.069.441691
AGGTTGT485450.069.3799310
GGTAGTA640650.069.364624
CCTTCAT283900.069.3533862
TGCTGGC141550.069.342689
TAGTAGG467200.069.310136
AGATGCT141600.069.299766
GTTGACA207200.069.2771618
AAGATGC141700.069.2765965
AGGTAGT640800.069.26753
AGCTTAT322150.069.266552
TATAGTT420100.069.2623216
GTTGTAT355500.069.2575512
CTTCATT284250.069.144353
GAGGTAG670800.069.1442
TGTATAG461950.069.1375514
GTTGTGT135450.069.13341512
Produced by FastQC (version 0.11.5)