FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6856630
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG938031.3680627363588236No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC409980.5979322203473134No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA257570.3756510122319565No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA218900.31925304413392586No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA217260.31686119857714357No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA216560.3158402888882731No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT190080.27722073380071555No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG181710.2650135708066499No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC156080.22763369176986362No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG137950.20119213082811815No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC119080.17367132250099537No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT111800.16305386173674238No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA97280.1418772779047433No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC85680.12495934591774675No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA72530.10578082819110847No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA72460.10567873722222142No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT518500.069.512878
TGAGGTA722300.069.487411
CCTTCAT359050.069.460412
AGTAGGT519650.069.4600457
AGATGCT176650.069.439256
TAGGTTG518800.069.438959
AGGTTGT543400.069.4121610
GTTGTAT402200.069.3941712
TAGTAGG520400.069.334926
AGGTAGT715600.069.319573
GGTAGTA717750.069.306964
AAGATGC177250.069.2644355
CGGAGTC358800.069.25384514
TGCTGGC176950.069.25369
AGCTTAT408050.069.250332
TATAGTT474800.069.24879516
TGTATAG521950.069.2344214
GCTGGCA177150.069.1951710
GTAGTAG713350.069.190395
GAGGTAG751500.069.153582
Produced by FastQC (version 0.11.5)