FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7212073
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG736851.0216896029754552No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC417980.5795559750989764No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA243020.3369627567552353No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG225470.3126285604707551No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT199550.27668882441983045No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG179770.24926259065874679No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC173190.2401390002569303No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC127910.1773553872790805No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT120240.1667204422362336No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA111960.15523969321996603No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA107720.14936066232274686No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA90080.12490167528808983No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC75980.10535112442705445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCAT311650.069.446212
GTAGGTT416000.069.3895348
GGAGTCT311950.069.32493615
AGTAGGT416850.069.323597
TAGGTTG416650.069.281289
AGGTTGT435950.069.2322510
CGGAGTC312800.069.21487414
GGTAGTA576750.069.19824
TGAGGTA580200.069.1900251
CTTCATT312100.069.153053
AGGTAGT576250.069.14383
GTTGTAT324750.069.13599412
TATAGTT384900.069.1149516
TAGTAGG418300.069.105776
AGATGCT138950.069.0607156
GTAGTAG573950.068.932135
TGCTGGC139200.068.905819
TGTATAG420650.068.89714
AAGATGC139350.068.862955
AGCTTAT286800.068.83722
Produced by FastQC (version 0.11.5)