FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7011281
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG909221.2967958351690654No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC450420.6424218341840813No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA290270.4140042311811493No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG260960.3722001728357485No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT237240.33836897993390935No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA199070.28392814380139664No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC172010.24533319945385157No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA147290.2100757336640765No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA140580.20050544258602673No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG137420.19599842026014935No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC132610.18913804766917772No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT123290.17584518435361526No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA94770.13516788158968382No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC94630.1349682033853728No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA74860.10677078839088036No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT461700.069.4984058
AGTAGGT462350.069.4536747
TAGGTTG462500.069.385269
AGATGCT157050.069.34676
CCTTCAT337150.069.340472
AGGTTGT484350.069.32651510
TGAGGTA666450.069.3138051
GTTGTAT362400.069.31052412
GGTAGTA660800.069.283754
CGGAGTC336700.069.2688314
TATAGTT438650.069.25999516
TAGTAGG463200.069.23746
GGAGTCT336550.069.1952415
AAGATGC157450.069.193255
GGATAGG80350.069.1584814
TGTATAG481950.069.1582214
AGGTAGT660900.069.1514743
CAAGATG157650.069.061074
GCTGGCA157650.069.0517110
TTGTATA482450.069.05028513
Produced by FastQC (version 0.11.5)