FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4302592
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC473051.0994535387041113No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG404190.9394104762896414No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT159630.3710089174153627No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC144700.3363089040280835No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC133480.31023159992860116No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA117750.27367224222050335No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG116920.27174317248765395No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA113480.26374799190813353No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG90530.21040805170464688No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT87820.2041095228178735No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA76980.17891540727078004No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA72110.16759664871779614No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA66400.15432557862795265No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG49350.11469830279050394No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT271300.069.550468
CCTTCAT185300.069.44782
AGTAGGT272450.069.372497
TAGGTTG271900.069.371249
GTTGTAT213900.069.3362212
AGGTTGT285000.069.2768510
TAGTAGG273000.069.262396
TGAGGTA390500.069.262121
TGCTGGC79150.069.1931849
CGGAGTC184900.069.17599514
AAGATGC79100.069.151665
TATAGTT260300.069.1294116
GGTAGTA387500.069.080114
AGGTAGT387150.069.026663
AGATGCT79200.069.0209666
CTTCATT186350.068.998543
GTAGTAG384400.068.963515
CCACCGG186300.068.955910
TGTATAG284200.068.94318414
AGCTTAT235100.068.9237062
Produced by FastQC (version 0.11.5)