FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6434727
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG696731.0827654382229424No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC618970.9619211506564304No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC224520.34891923153849413No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA220970.343402291969807No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG186570.2899423705154857No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC177490.27583143775951957No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT176460.2742307482508582No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT176190.27381115003014117No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG149010.23157159581129083No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA113620.17657314754767373No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA111080.17262581613796515No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA79150.12300444136946291No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA75570.11744087977625159No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG73640.1144415295318667No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA68430.10634483793951165No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT422300.069.388978
AGTAGGT422900.069.364997
TAGGTTG422350.069.3393259
CCTTCAT326150.069.2828452
AGATGCT147400.069.277696
CGGAGTC325500.069.265314
TAGTAGG423500.069.25456
TGAGGTA599400.069.2480851
GTTGTAT330650.069.21355412
AAGATGC147600.069.207525
GGTAGTA593050.069.199224
AGGTTGT442450.069.1788710
AGGTAGT592800.069.16993
CTTCATT325950.069.163773
GGAGTCT325200.069.13550615
TGCTGGC148000.069.110769
GCTGGCA148000.069.0161910
TATAGTT396850.069.0156416
AGCTTAT322700.069.015012
GAGGTAG619550.069.008172
Produced by FastQC (version 0.11.5)