FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6592013
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG640540.9716910449054029No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC428670.6502869457326617No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA418580.6349805438793886No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA393180.5964490664687706No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA217370.3297475293207098No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG202900.30779672309505457No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT178240.27038781628616326No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA161590.2451299777473133No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG150080.22766945392856475No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC142910.21679265499021314No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA142450.21609484083238306No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT138420.20998138201487163No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC129820.1969352912380482No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA80130.12155619231940228No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT419000.069.4330148
TGAGGTA580650.069.3680341
AGTAGGT419550.069.358157
TAGGTTG419250.069.3498849
TAGTAGG420000.069.272956
AGGTTGT440100.069.26861610
CCTTCAT257400.069.25642
GTTGTAT322300.069.2138812
GTTGTGT122300.069.1549812
GGTAGTA576650.069.1470264
TATAGTT381500.069.12253616
AGATGCT119000.069.1072856
CTTCATT257550.069.080193
TGTATAG418100.069.03062414
CAAGATG119250.068.99284
TGCTGGC119750.068.9906859
GTAGTAG574450.068.9056855
AAGATGC119500.068.877235
GGTTGTG123000.068.8752211
TTGTATA418750.068.823213
Produced by FastQC (version 0.11.5)