FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7929739
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG912181.1503278985600913No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC683660.8621469130320683No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA319220.4025605382472235No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA295970.3732405316240547No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG293770.3704661654059484No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA280720.35400912943036333No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT260710.32877500760113293No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT234000.2950916795622151No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC221770.2796687255406515No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC211350.26652831827125706No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160150.20196124992260148No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA126290.15926123167483822No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA121150.15277930332889897No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC105630.13320741073571274No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA91580.11548929920644298No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT505350.069.438888
TGAGGTA722200.069.365981
AGTAGGT507000.069.364747
AGATGCT151800.069.349646
AGGTTGT530000.069.3058710
CCTTCAT331250.069.301372
GTTGTAT386950.069.2986312
TAGTAGG507400.069.2985466
TAGGTTG506850.069.226049
GGTAGTA716000.069.213994
CTTCATT331450.069.119673
TATAGTT473750.069.1069416
AAGATGC152450.069.0773545
TGTATAG517350.069.0182514
CAAGATG152550.069.009574
AGCTTAT317950.068.963992
CGGAGTC332250.068.92174514
GCTGGCA153150.068.9120210
GTAGTAG713800.068.887545
GGATAGG72600.068.876914
Produced by FastQC (version 0.11.5)